• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文OA文献 >Issues encountered in development of enzymelinked immunosorbent assay for use in detecting \u3ci\u3eInfluenza A virus\u3c/i\u3e subtype H5N1 exposure in swine
【2h】

Issues encountered in development of enzymelinked immunosorbent assay for use in detecting \u3ci\u3eInfluenza A virus\u3c/i\u3e subtype H5N1 exposure in swine

机译：酶联开发中遇到的问题 免疫吸附试验用于 检测\ u3ci \ u3eInfluenza a virus \ u3c / i \ u3e亚型H5N1 在猪身上暴露

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A potential mechanism by which highly pathogenic avian Influenza A virus subtype H5N1 could more readily infect human beings is through the infection of and adaptation in pigs. To detect the occurrence of such infection, monitoring of pig populations through serological screening would be highly desirable. In the current study, hemagglutination inhibition assays were able to detect antibodies against H5N1 developed in pigs, but because of antigenic variation between clades, the use of multiple virus strains were required. Whole recombinant virus and recombinant hemagglutinin antigen enzymelinked immunosorbent assays (ELISAs) were generated that could detect antibody against multiple H5N1 strains, but which also detected antibody against endemic swine influenza viruses. A recombinant hemagglutinin antigen-based ELISA was as effective as the whole virus antigen ELISAs in detecting antibody against the H5N1 virus strains used and eliminated nearly all of the cross-reactivity with non-H5N1 virus antibody. The current study also highlighted the difficulty in establishing a decision (cutoff) value that would effectively counterbalance nonspecific reactivity against sensitivity. The results provide important information and considerations for the development of serological screening assays for highly pathogenic avian H5N1 viruses.
机译：高致病性禽流感H5N1亚型可以更容易感染人类的​​潜在机制是通过猪的感染和适应。为了检测这种感染的发生，非常需要通过血清学筛查来监测猪的数量。在当前的研究中，血凝抑制试验能够检测出针对猪的H5N1抗体，但是由于进化枝之间的抗原差异，需要使用多种病毒株。产生了完整的重组病毒和重组血凝素抗原酶联免疫吸附试验（ELISAs），可以检测针对多种H5N1毒株的抗体，但也可以检测针对地方性猪流感病毒的抗体。基于重组血凝素抗原的ELISA在检测针对所用H5N1病毒株的抗体方面与整个病毒抗原ELISA一样有效，并且几乎消除了与非H5N1病毒抗体的所有交叉反应性。当前的研究还强调了建立决策（临界）值的困难，该值将有效平衡非特异性反应性和敏感性。结果为开发高致病性禽H5N1病毒的血清学筛选测定法提供了重要的信息和考虑因素。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号